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CIK cells may offer some advantages over other cell therapy products, including ease of in vitro propagation and no need for exogenous administration of IL 2 for in vivo priming. NK cells and CIK cells can be expanded using a variety of clinical grade approaches, before their infusion into patients with cancer. Herein, we discuss GMP compliant strategies to isolate and expand human NK and CIK cells for immunotherapy purposes, focusing on clinical trials of adoptive transfer to patients with hematological malignancies..

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